Genotyping By Sequencing (GBS)
Genotyping by sequencing (GBS) is a simple highly-multiplexed system for constructing reduced representation libraries for the Illumina next-generation sequencing platform developed in the Buckler lab by Rob Elshire. It generates large numbers of single nucleotide polymorphisms (SNPs) for use in genetic analyses. Key components of this system are: reduced sample handling, fewer PCR and purification steps, no size fractionation and inexpensive barcoding. We use restriction enzymes to reduce genome complexity and avoid the repetitive fraction of the genome. The result is tens to hundreds of thousands of genotyped SNP markers, ready to analyze!
We have successfully conducted GBS on many organisms, including: maize, sorghum, cassava, willow, rice, apples, cow, fox, and more!
Find out about our next GBS workshop
Get our protocol
Please note: this is a new dilution for the new v3 of the TruSeq SR Cluster Kit. If you are using a different enzyme or your sequencing facility is using the earlier v2 you need to adjust your dilution. Please contact firstname.lastname@example.org).
Using Qiagen DNeasy columns is our preferred DNA extraction protocol for GBS samples, download it here