The Biotechnology Centre (UG) & The Institute for Genomic Diversity (Cornell University)
Training Course on the Use of Molecular Markers for
Characterization of Genetic Resources
30 June – 11 July 2008
Agenda
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Lectures, Seminars, & Laboratories |
Location, Lead person |
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30 June Monday |
09:00 |
Welcoming Remarks |
Sam Offei
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Group introductions |
T Fulton |
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10:30 |
Break |
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10:45 |
Schedule, materials, expectations Introduction Plant Genetic Diversity & evaluation About genetic markers, importance of Properties of markers, choosing of DNA basics, restriction enzymes & DNA-based markers |
T Fulton |
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12:00 |
Lunch |
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01:30 |
Lab Safety, Use of equipment DNA grinding and CTAB extraction DNA extraction w/FTA paper system |
Team
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?? |
Cocktail reception |
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1 July |
09:00 |
Make DNA quantification agarose gel |
Team |
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Tuesday |
09:30 |
The Polymerase Chain Reaction & PCR-based markers PCR techniques with arbitrary primers Sequence-tagged site markers: Microsatellites, CAPS, others |
T Fulton |
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10:30 |
Break |
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11:00 |
Load & start quantification gel |
Team |
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12:00 |
Lunch |
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01:30 |
Demo of horizontal PAGE and about KT |
Ed Southern |
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02:30 |
Break |
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03:00 |
Applying MAS in cocoa breeding and other research in Ghana: challenges and role of the Mobile Laboratory |
Jemmy Takrama |
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04:00 |
Stain gel, check DNA, quantify Discussion |
Team S Mitchell, T Fulton |
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2 July Wed |
09:00 |
PCR with SSRs Prepare gel |
Team |
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10:30 |
Break |
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11:00 |
AFLP markers |
S Mitchell
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12:30 |
Lunch |
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01:30 |
Genetic mapping |
R Koebner |
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03:00 |
Break |
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03:30 |
Load gel Question and Answer session |
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3 July Thursday |
09:00 |
Stain gel, take picture |
Team |
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09:30 |
Genetic Diversity & use of markers |
S Mitchell |
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10:30 |
Break |
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11:00 |
Analyzing SSR results |
S Mitchell |
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12:00 |
Lunch |
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01:30 |
Use of databases: Sequence Retrieval etc Primer design Web Resources |
Computer Lab T Fulton |
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Break where convenient |
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Literature Team assignments (flexible) |
T Fulton |
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4 July Friday |
09:00 |
New technologies: DNA sequencing, ESTs, SNPs, Comparisons of markers, Considerations in choosing, Cost-saving strategies |
T Fulton |
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10:30 |
Break |
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11:00 |
Proposal Writing |
T Fulton |
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12:00 |
Lunch |
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01:30 |
Marker-assisted selection: Principles and applications |
R Koebner |
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03:00 |
Break |
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03:30 |
QTL identification & use in industry: case study in tomato |
T Fulton |
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04:30 |
Problems, questions, discussion |
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5 July Saturday |
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Field trip to Cape Coast/Elimina Castles/Canopy Walk?
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6 July Sunday |
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Free time
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7 July Monday |
09:00 |
Phenotyping: Methods, experimental design & statistical analyses used in breeding programs |
T Fulton |
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10:00 |
Break |
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10:30 |
Molecular Cloning: Library construction |
S Mitchell |
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12:00 |
Lunch |
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01:30 |
Ligation & Transformation of E. Coli |
Team |
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03:00 |
Break |
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03:30 |
Plating? PAGE? |
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8 July Tuesday |
09:00 |
Proteomics & other ÔomicsÕ: whatÕs on the horizon |
T Fulton |
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10:00 |
Break |
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10:30 |
Marker-assisted selection: complications of polyploidy |
R Koebner |
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12:00 |
Lunch |
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01:30 |
Strategic banana and plantain improvement project at IITA: status and way forward |
Irie Vroh Bi (IITA) |
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03:00 |
Break |
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03:30 |
Manuscript writing, Poster Design |
R Koebner |
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9 July |
09:00 |
Team Presentations |
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Wed |
10:30 |
Break |
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11:00 |
Team Presentations, contd. |
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12:00 |
Lunch |
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01:30 |
Team Presentations Discussion of use of markers, Question & Answers |
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03:00 |
Break |
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03:30 |
Seminar: Identification of a MADS-box gene family and phylogenetic analysis of the mikc subgroup in cowpea |
Joseph Opoku |
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10 July |
09:00 |
Seminar?? |
??? Walter Alhassan? |
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Thurs |
10:30 |
Break |
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11:00 |
Course evaluations |
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12:00 |
Lunch |
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01:30 |
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Wrap-up, questions |
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Group Dinner |
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