The Biotechnology Centre (UG) & The Institute for Genomic Diversity (Cornell University)
Training Course on the Use of Molecular Markers for
Characterization of Genetic Resources
2 July – 13 July 2007
Tentative Agenda
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Lectures, Seminars, & Laboratories |
Location, Lead person |
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2 July Monday |
09:00 |
Welcoming Remarks |
Sam Offei Robert Koebner |
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Group introductions |
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10:30 |
Break |
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10:45 |
Schedule, materials, expectations Introduction Plant Genetic Diversity & evaluation About genetic markers, importance of Properties of markers, choosing of DNA basics, restriction enzymes & DNA-based markers |
T Fulton |
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12:00 |
Lunch |
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01:30 |
Lab Safety, Use of equipment DNA grinding and CTAB extraction DNA extraction w/FTA paper system |
Team R Koebner |
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?? |
Cocktail reception |
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3 July |
09:00 |
Make DNA quantification agarose gel |
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Tuesday |
09:30 |
The Polymerase Chain Reaction & PCR-based markers PCR techniques with arbitrary primers |
T Fulton |
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10:30 |
Break |
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11:00 |
Load & start quantification gel |
Team |
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12:00 |
Lunch |
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01:00 |
AFLP markers |
S Mitchell |
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02:00 |
Seminar: MAS in animal breeding -current and future applications |
B Kayan |
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03:00 |
Break |
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03:30 |
Stain gel, check DNA, quantify Discussion |
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4 July Wed |
09:00 |
Sequence-tagged site markers: Microsatellites, CAPS, others |
T Fulton |
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10:30 |
Break |
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11:00 |
Basics of linkage mapping Software available for linkage mapping |
R Koebner |
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12:00 |
Lunch |
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01:30 |
Discussion of AFLP procedures Start AFLPs |
S Mitchell R Koebner |
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03:00 |
Break |
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03:30 |
Marker-assisted selection: Principles and applications, complications of polyploidy |
R Koebner |
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5 July Thursday |
09:00 |
AFLPs |
S Mitchell Lab staff |
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10:30 |
Break |
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11:00 |
Manuscript writing (timing tentative) |
R Koebner |
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12:00 |
Lunch |
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01:30 |
AFLPs continued Prepare AFLP-silver staining gel |
S Mitchell |
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03:00 |
Break |
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03:30 |
Seminar: Yam Breeding |
Robert Asiedu (IITA) |
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6 July |
09:00 |
Load AFLP gel Protein gel demonstration |
R Koebner, S Mitchell, Team |
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Friday |
10:30 |
Break |
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11:00 |
Phenotyping: Methods, experimental design & statistical analyses used in breeding programs |
Kwadjo Ofori T Fulton |
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12:00 |
Lunch |
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01:30 |
Seminar: African Biotechnology and Biosafety Initiative: Stakeholders perspectives on Regional Biotechnology Networking and Coordination |
FARA |
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03:00 |
Break |
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03:30 |
Gel visualization Problems, questions, discussion |
R Koebner S Mitchell |
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Questions, evaluation |
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7 July Saturday |
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Field trip to Cape Coast/Elimina Castles/Canopy Walk?
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8 July Sunday |
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Free time
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9 July Monday |
09:00 |
PCR with SSR primers
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S Mitchell |
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10:00 |
Break |
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10:30 |
QTL identification & use in MAS Pitfalls, expectations, use across species |
T Fulton |
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12:00 |
Lunch |
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01:30 |
Prepare gel for SSR detection |
Team T Fulton |
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03:00 |
Break |
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03:30 |
Start gel Question/answer session |
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10 July Tuesday |
09:00 |
Stain gel Discussion of results |
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10:00 |
Break |
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10:30 |
Plant Pathology |
Sam Offei |
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12:00 |
Lunch |
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01:30 |
Use of databases: Sequence Retrieval etc Primer design |
Computer lab? T Fulton/ S Mitchell |
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(break where convenient) |
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Web Resources for Scientists: Resource Center, Agora, etc. |
T Fulton |
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11 July Wed |
09:00 |
New technologies: DNA sequencing, ESTs, SNPs, Microarrays |
T Fulton |
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10:30 |
Break |
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11:00 |
Proteomics & other ÔomicsÕ, an overview of whatÕs on the horizon |
T Fulton |
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12:00 |
Lunch |
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01:30 |
Molecular Cloning: Library construction |
S Mitchell |
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02:30 |
Ligation & Transformation of E. Coli |
S Offei |
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12 July |
09:00 |
Team Presentations |
T Fulton |
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Thurs |
10:30 |
Break |
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11:00 |
Team Presentations, contd. |
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12:00 |
Lunch |
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01:30 |
Team Presentations, wrap-up and discussion |
Team |
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02:30 |
Break |
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03:00 |
Measures of genetic diversity & use of markers, Software available |
S Mitchell |
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04:00 |
Comparisons of markers, Considerations in choosing Cost-saving strategies |
T Fulton Discussion |
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13 July |
09:00 |
Proposal Writing |
T Fulton |
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Friday |
10:30 |
Break |
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11:00 |
Facilities available in W. Africa, opportunities for collaborations and outsourcing, funding opportunities |
W Alhassan |
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12:00 |
Lunch |
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01:30 |
Poster design |
R Koebner |
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Wrap-up, questions |
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Course evaluations |
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Group Dinner |
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